American Society for Clinical Pathology Board of Certification (ASCP BOC) · Specialist in Molecular Biology—SMB(ASCP) and SMB(ASCPi), Examination Content Guideline, revised September 25, 2025. The document states a revision date but no separate effective date. The workbook label SMB maps to the official Specialist in Molecular Biology credential; the guideline covers both ASCP and ASCPi pathways.

This original 20-question sample covers selected topics across the outline. It does not reproduce official exam questions or cover every tested topic.

• Specialist in Molecular Biology—SMB(ASCP) and SMB(ASCPi): Examination Content Guideline
• NHGRI: Ribonucleic Acid Fact Sheet
• NHGRI: Autosomal Recessive Disorder
• Thermo Fisher Scientific: TaqMan vs. SYBR Chemistry for Real-Time PCR
• Applied Biosystems: Amplification Efficiency
• Thermo Fisher Scientific: What Is Sanger Sequencing?
• MRC Holland: Is It Possible to Detect SNVs with MLPA and digitalMLPA?
• Illumina: Sequencing Quality Scores
• Illumina DRAGEN: QC Metrics and Coverage/Callability Reports
• QIAGEN CLC Genomics Workbench: Detecting DNA Methylation
• CDC: Good Laboratory Practices for Molecular Genetic Testing for Heritable Diseases and Conditions
• CMS: CLIA Verification of Performance Specifications
• CMS: Proficiency Testing and PT Referral
• NIST: Coefficient of Variation
• FDA: Statistical Guidance on Reporting Results from Studies Evaluating Diagnostic Tests
• FDA: Establishing Performance Characteristics of Devices for Detection or Differentiation of Influenza Viruses
• FDA: Commercially Distributed Analyte Specific Reagents—Frequently Asked Questions
• Association for Molecular Pathology: Molecular Diagnostic Assay Validation White Paper
• European LeukemiaNet: Laboratory Recommendations for Diagnosis and Management of Chronic Myeloid Leukemia
• ARUP Laboratories: Angelman Syndrome and Prader-Willi Syndrome by Methylation-Specific MLPA
• CDC: Clinical Testing and Diagnosis for C. diff Infection
• ARUP Laboratories: Chimerism Testing by Short Tandem Repeat Genotyping
• CPIC Guideline for CYP2C19 Genotype and Clopidogrel Therapy: 2022 Update
• National Cancer Institute: BRCA Gene Changes—Cancer Risk and Genetic Testing
• ARUP Laboratories: FMR1 Premutation Example Report
• NMDP: HLA Reporting Background
• ASCP BOC: Earn a Credential
• NHGRI: Talking Glossary of Genomic and Genetic Terms
• New England Biolabs: Isothermal Amplification and Strand Displacement
• Hologic: Aptima HPV 16 18/45 Genotype Assay—Instructions for Use, AW-26281-001 Rev. 001
• Thermo Fisher Scientific: QuantiGene Assays for Analysis of Gene Expression Utilizing Branched DNA Technology
• Thermo Scientific: Shake 'n Stack Hybridization Oven Operating and Maintenance Manual
• NHGRI: Fluorescence In Situ Hybridization Fact Sheet
• CMS: CLIA Assessing Personnel Competency
• OSHA: Laboratories—Overview
• MedlinePlus Genetics: HBA2 Gene
• MedlinePlus Genetics: HBB Gene
• MedlinePlus Genetics: F5 Gene
• MedlinePlus Genetics: F2 Gene
• MedlinePlus Genetics: MTHFR Gene
• NHGRI: Researchers Examine Disease-Causing Mutations in Mitochondrial Genomes